A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Table 2 Automated solution hybrid selection performance

Performance factor	3 μg input average (n= 1,117 exomes)
Median target coverage	131.0×
Percentage bases > 2×	96.0%
Percentage bases > 10×	91.9%
Percentage bases > 20×	87.6%
Percentage selected bases (on target)	83.7%
Percentage duplicated reads	4.4%
Fold 80 penalty^a	3.17
Estimated library size of captured fragments	278 million

See Additional file 12 for metric definitions. ^aFold 80 penalty is a measure of the non-uniformity of sequence coverage, defined as the amount of additional coverage (in fold coverage of the genome) required so that 80% of the target bases will be covered at the current mean coverage (see Additional file 12 for details).

ISSN: 1474-760X