Comparison of the fraction of biclusters with significant GO and KEGG annotation enrichments for the single and multi-species methods for the B. subtilis - B. anthracis pairing. (a) GO terms. For all multi-species methods there is a consistent increase from the shared to elaboration step, with the percentage of elaborated biclusters with significant GO term enrichments consistently greater than those from the single species optimization. (b) KEGG pathways. For both of the multi-species biclustering methods (MSCM and MSISA), there is a consistent increase in percentage from the shared to elaborated optimizations, similar to the GO term enrichments, with a similarly large increase for the refined MSISA biclusters for B. anthracis. The two k-means clustering variants showed either negligible increase or even a decrease between the shared and elaboration steps.