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Figure 10 | Genome Biology

Figure 10

From: Genome-wide analysis of mRNA decay patterns during early Drosophiladevelopment

Figure 10

Effects of miR-14 on mRNA expression during early Drosophila embryogenesis. (a) Hr78 mRNAs suffered degradation during the first 3 h of development. Microarray time course data (compare Figure 1a). (b) Hr78 mRNAs are predicted to be targeted by five miRNAs, including miR-14 (MicroCosm [112]). (c) Lowering the dose of miR-14 led to significant stabilization of Hr78 mRNAs in early embryos. Semi-quantitative RT-PCR experiments for Hr78 were carried out on RNA samples from wild-type (+/+) and embryos heterozygous (ΔmiR-14/+) or homozygous (ΔmiR-14/ΔmiR-14) for a miR-14 deletion. Lowering the dose of miR-14 led to stabilization of Hr78 mRNAs in a dose-dependent manner. Hr78 signals in agarose gels were normalized to RpL32 (aka Rp49) signals; gels were analyzed using ImageJ software. Error bars, standard error of the mean (SEM).

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