Figure 1

Genome-wide expression profiles in early Drosophila embryos and unfertilized eggs. (a) Microarray time course. Experimental design: sampling intervals, morphological features of embryos, cell cycles (black bars), developmental stages after Hartenstein [111] (grey bars) and hallmarks of early fly development (grey boxes) are indicated. Confocal embryo images: DAPI/FITC-phalloidin stain to highlight cell nuclei (blue) and cell cortices (actin, red). Four replicate samples were analyzed for each treatment. (b) Microarray data quality assessment. Hierarchical clustering (Pearson correlation distance) grouped 24 microarrays (x-axis) into 6 replicate groups (see (a)). Expression levels for approximately 19,000 probe sets (y-axis) are shown in relation to median expression for each probe set across all microarrays. (c) Sample microarray expression profiles. Median log2 expression of four biological replicates; 1 Unit = log2 fold-change 1; error bars represent standard error of the mean over replicates.