The influence of read-depth on discovering personal genomic variants. Sequences included in our analysis are represented by blue squares, with names or codenames as shown in Figure 1. The lines indicate correlations; the YH genome (red square) was not included in the correlation analysis because it is thought to be an outlier, possibly owing to its higher percentage of single-end-read sequencing. (a) Higher read-depth sequencing can reveal more SNPs, as shown by the positive correlation between read-depth and number of SNPs detected. (b) The ratio of heterozygous to homozygous SNPs shows a positive relationship with read-depth of sequencing, suggesting that high-depth sequencing can detect heterozygous SNPs better.