Figure 1

Strand-specific RNA-seq identifies 1,103 antisense units associated with stationary phase, stress, and meiosis genes in S. cerevisiae. (a) Typical short antisense (Unit3689, antisense to NOP10). Shown are reads mapped from a standard cDNA sequencing library [15] (yellow), and from the strand-specific library prepared and run side-by-side on the same flow cell (green: forward reads above, reverse reads below). All coverage tracks were normalized to the total number of reads mapped, and are shown up to a threshold of 3 × 10^-8 of total mapped reads (genome-wide). Units were called from the strand-specific library (blue units, known genes; orange, putative antisense), and are shown along with the manually curated units (red) and the known gene annotations from the SGD (gray). (b) Typical long antisense (ManualUnit225, antisense to MBR1). Tracks are as in (a). The figures are shown using the Integrative Genome Viewer [36].