Digital expression profiling of novel diatom transcripts provides insight into their biological functions

Table 2 Top 20 most highly expressed cDNAs across all the libraries, and their presence in different genomes

Contig	Cluster size^a	G^b	BLASTX description	InterPro description
G49202	2,079	P	-	-
G55010	856	P	-	Pyridoxal phosphate-dependent decarboxylase
G47667	833	O	Solute carrier family 34	Na+/Pi cotransporter
G27877	658	O	Ammonium transporter	Rh-like protein/ammonium transporter
G13951	630	C	Glutamate dehydrogenase	Glutamate dehydrogenase
G51797	613	D	Alpha 3 frustulin	-
G52619	605	O	Uric acid-xanthine permease	Xanthine/uracil/vitamin C permease
G44694	586	D	M6 family	Aldehyde dehydrogenase
G20424	561	O	Urea active isoform	Na+/solute symporter
G48027	545	P	-	-
G48315	479	V	Choline carnitine betaine transporter	BCCT transporter
G176.1	463	O	Alternative oxidase	Alternative oxidase
G29456	379	C	Glyceraldehyde-3- phosphate dehydrogenase	Glyceraldehyde 3-phosphate dehydrogenase
G49064	358	H	-	Na+/H+ antiporter NhaC
G49151	353	D	Nucleoside diphosphate epimerase	NmrA-like
G49211	346	P	-	-
C358	344	V	Periplasmic l-amino acid catalytic subunit	-
G30648	342	V	Light harvesting protein	Chlorophyll A-B binding protein
G23629	333	C	Calcium transporting ATPase	E1-E2 ATPase-associated region
G45835	316	V	-	Sterol-sensing 5TM box

^aCluster size of contig. ^bConserved in different representatives from eukaryotic genomes (e-value cutoff 10^-5; more than 30% identity and 50% coverage): C, core (plant/animal/diatom); D, diatom (P. tricornutum and T. pseudonana); O, animal (opisthokonts); P, Phaeodactylum tricornutum; V, plant (Viridiplantae).

ISSN: 1474-760X