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Table 1 List of different libraries and culture conditions together with library statistics

From: Digital expression profiling of novel diatom transcripts provides insight into their biological functions

Library Short name Strains Condition/mediuma cDNAs Contigs Singletons TUs %Rb
Original standardc OS Pt1 clone 8.6 (CCAP1055/1)   12,136 3,274 1,165 4,439 67.31
Silica plus SP Pt1 clone 8.6 (CCAP1055/1) 350 uM metasilicate in ASW 7,508 3,077 384 3,461 57.21
Silica minus SM Pt1 clone 8.6 (CCAP1055/1) No metasilicate addition 6,968 2,838 459 3,297 54.63
Oval morphotype OM Pt3 (CCAP1052/1B) Low salinity (10% ASW) 4,544 2,202 214 2,416 48.78
Nitrate replete NR Pt1 clone 8.6 (CCAP1055/1) 1.12 mM in chemostat 3,632 2,028 242 2,270 39.01
Nitrate starved NS Pt1 clone 8.6 (CCAP1055/1) 50 μM for 3 days in chemostat 9,122 3,271 512 3,783 60.79
Ammonium adapted AA Pt1 clone 8.6 (CCAP1055/1) 75 μM 9,031 3,329 567 3,896 60.20
Urea adapted UA Pt1 clone 8.6 (CCAP1055/1) 50 μM 8,552 3,157 464 3,621 59.82
Tropical accession TA Pt9 (CCMP633) Grown at 15°C 4,821 2,015 160 2,175 56.95
Low decadienal LD Pt1clone 8.6 (CCAP1055/1) 0.5 μg/m 2E,4E-decadienal for 6 h 9,227 3,322 537 3,859 61.65
High decadienal HD Pt1 clone 8.6 (CCAP1055/1) 5 μg/m 2E,4E-decadienal for 6 h 3,541 1,734 323 2,057 44.95
Iron limited FL Pt1 clone 8.6 (CCAP1055/1) 5 nM 8,264 3,064 487 3,551 59.19
Triradiate morphotype TM Pt8 (CCAP1055)   12,566 3,055 520 3,575 70.49
Blue light BL Pt1 clone 8.6 (CCAP1055/1) 48 h dark adapted cells exposed to 1 h blue light 12,045 4,253 607 4,860 59.61
CO2 high 4 days C4 Pt1 clone 8.6 (CCAP1055/1) 3.2 mM DIC for 4 days in chemostat 10,283 3,564 160 3,724 63.78
CO2 high 1 day C1 Pt1 clone 8.6 (CCAP1055/1) 3.2 mM DIC for 1 day in chemostat 10,307 3,598 165 3,763 63.49
  1. aAll cells grown in artificial seawater media, except chemostat cultures, which were grown in Walne medium [54]. bPercent redundancy of sequences in each library. cThe original P. tricornutum cDNA library described previously [21, 22] is herein referred to as OS. Although incorporated into the comparative expression analyses, it was not examined extensively because it was generated using a different cDNA library protocol. ASW (artificial sea water),; TU, transcriptional unit.
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Genome Biology

ISSN: 1474-760X

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