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Table 1 List of different libraries and culture conditions together with library statistics

From: Digital expression profiling of novel diatom transcripts provides insight into their biological functions

Library

Short name

Strains

Condition/mediuma

cDNAs

Contigs

Singletons

TUs

%Rb

Original standardc

OS

Pt1 clone 8.6 (CCAP1055/1)

 

12,136

3,274

1,165

4,439

67.31

Silica plus

SP

Pt1 clone 8.6 (CCAP1055/1)

350 uM metasilicate in ASW

7,508

3,077

384

3,461

57.21

Silica minus

SM

Pt1 clone 8.6 (CCAP1055/1)

No metasilicate addition

6,968

2,838

459

3,297

54.63

Oval morphotype

OM

Pt3 (CCAP1052/1B)

Low salinity (10% ASW)

4,544

2,202

214

2,416

48.78

Nitrate replete

NR

Pt1 clone 8.6 (CCAP1055/1)

1.12 mM in chemostat

3,632

2,028

242

2,270

39.01

Nitrate starved

NS

Pt1 clone 8.6 (CCAP1055/1)

50 μM for 3 days in chemostat

9,122

3,271

512

3,783

60.79

Ammonium adapted

AA

Pt1 clone 8.6 (CCAP1055/1)

75 μM

9,031

3,329

567

3,896

60.20

Urea adapted

UA

Pt1 clone 8.6 (CCAP1055/1)

50 μM

8,552

3,157

464

3,621

59.82

Tropical accession

TA

Pt9 (CCMP633)

Grown at 15°C

4,821

2,015

160

2,175

56.95

Low decadienal

LD

Pt1clone 8.6 (CCAP1055/1)

0.5 μg/m 2E,4E-decadienal for 6 h

9,227

3,322

537

3,859

61.65

High decadienal

HD

Pt1 clone 8.6 (CCAP1055/1)

5 μg/m 2E,4E-decadienal for 6 h

3,541

1,734

323

2,057

44.95

Iron limited

FL

Pt1 clone 8.6 (CCAP1055/1)

5 nM

8,264

3,064

487

3,551

59.19

Triradiate morphotype

TM

Pt8 (CCAP1055)

 

12,566

3,055

520

3,575

70.49

Blue light

BL

Pt1 clone 8.6 (CCAP1055/1)

48 h dark adapted cells exposed to 1 h blue light

12,045

4,253

607

4,860

59.61

CO2 high 4 days

C4

Pt1 clone 8.6 (CCAP1055/1)

3.2 mM DIC for 4 days in chemostat

10,283

3,564

160

3,724

63.78

CO2 high 1 day

C1

Pt1 clone 8.6 (CCAP1055/1)

3.2 mM DIC for 1 day in chemostat

10,307

3,598

165

3,763

63.49

  1. aAll cells grown in artificial seawater media, except chemostat cultures, which were grown in Walne medium [54]. bPercent redundancy of sequences in each library. cThe original P. tricornutum cDNA library described previously [21, 22] is herein referred to as OS. Although incorporated into the comparative expression analyses, it was not examined extensively because it was generated using a different cDNA library protocol. ASW (artificial sea water),; TU, transcriptional unit.