Figure 7
From: Identification of novel exons and transcribed regions by chimpanzee transcriptome sequencing
Example of an intergenic novel transcript that was absent in the human genome. (a) A view from the UCSC Genome Browser showing an intergenic region on chromosome 1. Two novel TRs are shown as blue boxes and the position of primers and the experimentally validated fragments are indicated with lines. (b) Results of the experimental validation with RT-PCR. The first two gels show fragments covering the two novel TRs and the third gel is a fragment spanning the junction between the novel TRs. The first lane on the gels is a 100-bp ladder (molecular weight marker, MWM), then follows the brain sample with (+RT) and without (-RT) reverse transcriptase, followed by the same set of experiments in the liver sample.