Figure 1

Work flow for the bioinformatics analyses. Sequence reads were mapped to the reference genome (PanTro2), a coverage signal was calculated across the genome and a threshold for expression was established. The threshold was initially used to determine expression of RefSeq genes and later for de novo detection of TRs. TRs with no previous annotations were considered to be novel and further characterized. De novo prediction of splice junctions was performed to join novel TRs with each other and with existing gene models.