Figure 4

Evolutionary constraint of AK082467 and Rmst. (a) The genomic region of mouse chromosome 10 (chr 10) encompassing lncRNAs AK082467 (2.7 kb) and Rmst (2.7 kb) is depicted. Note the presence of the protein-coding gene Nedd1 (neural precursor expressed developmentally down-regulated protein 1) upstream of AK082467 and Rmst. (b) A more detailed representation of AK082467 and Rmst (exons highlighted in yellow and orange, respectively), microRNAs mir-1251 and mir-135a-2, and their immediate flanking regions. Below the gene structures are the positions of H3K4me3 (green) and H3K27me3 (red) chromatin marks detected in mouse brain (obtained from the UCSC Genome Browser) as well as similar tracks as those in Figure 2b. Note the detected homology with orthologous frog sequence in Rmst exons 1, 2, 4, and 11. (c) Conservation and relative sizes of AK082467 and Rmst orthologs in various species. Note the conserved splice sites (green bars) in mouse Rmst exons 1, 4, and 11 as well as the sequence conservation (relative to mouse sequence) in exons 1 and 11, but differences in total exon number among species. The 3' ends of opossum and chicken orthologs have not been experimentally verified. Other details are provided in the legend to Figure 2. ECR, evolutionarily conserved region.