Figure 4
From: Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage
Oocyte arrest after ICSI with sperm from mice with NPY deficiency. Epididymal sperm from males with severe NPYq deficiency (9/10NPYq- and NPYq-2) but not males with moderate NPYq gene loss (2/3NPYq-) led to increased incidence of oocyte arrest compared to epididymal sperm from their appropriate controls, as revealed by Fisher's exact probability test. Statistical significance: a = P < 0.05; c = P < 0.001 versus matching sperm type in control. Epididymal sperm from males with severe NPYq deficiency (9/10NPYq- and NPYq-2) but not males with moderate NPYq gene loss (2/3NPYq-) led to increased incidence of oocyte arrest compared to testicular sperm, as revealed by Mantel-Haenszel chi square analysis. Statistical significance: **P < 0.01; ***P < 0.001. Two males were used per genotype; four sperm groups (epididymal, frozen epididymal, testicular and frozen testicular) were examined per male; approximately 20 (20.35 ± 6.94) oocytes were scored per group per male.
