A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes

Table 1 Gene Ontology enrichment analysis for six out of seven effective genes whose overexpression perturbed the mES transcriptome in a statistically significant manner

Gene	Gene Ontology term	FDR	Fold enrichment	Reference
Runx1	Negative regulation of progression through cell cycle	2.8	1.5	[60, 61]
	Positive regulation of cell proliferation	0.5	1.5	[60, 61]
	Vasculature development	0.1	1.5	[62, 63]
	Blood vessel development	0.2	1.5	[62, 63]
	Blood vessel morphogenesis	0.1	1.6	[62, 63]
	Angiogenesis	0.1	1.6	[62, 63]
	Regulation of myeloid cell differentiation	0.8	2.1	[63]
	Skeletal development	0.2	1.5	[64]
	Skeletal morphogenesis	1.7	2.5	[64]
	Regulation of cell differentiation	0.0	1.7	[65]
Erg	Anatomical structure formation	0.2	1.5	[66]
	Angiogenesis	0.0	1.6	[67, 68]
	Regulation of cell differentiation	0.8	1.5	[67, 68]
	Cell growth	2.3	1.5	[67, 68]
	Regulation of cell migration	1.3	1.8	[67, 68]
	Negative regulation of transcription, DNA-dependent	0.5	1.5	[68]
Nrip1	Muscle cell differentiation	2.0	4.3	[69]
	Nervous system development	0,1	2.0	[70]
	Negative regulation of transcription, DNA-dependent	3.6	2.6	[71]
Olig2	Organ morphogenesis	0.5	1.6	[72, 73]
	Placenta development	1.3	4.3	[74]
	Negative regulation of progression through cell cycle	3.6	2.1	[75]
Pdxk	Cellular macromolecule catabolic process	2.0	2.7	[76, 77]
	Cellular carbohydrate metabolic process	0.0	4.1	[76, 77]
	Amino acid biosynthetic process	0.6	7.3	[78]
Aire	Cell morphogenesis	0.0	1.6	[79]
	Regulation of progression through cell cycle	0.0	1.7	[79]
	Regulation of cell differentiation	0.4	1.9	[79]
	Cell migration	4.0	1.5	[80]

Perturbation of the mES transcriptome was as assessed by microarray analysis. GO analysis was performed on the list of differentially expressed genes using the DAVID tool, restricting the output to biological process terms of levels 4 and 5, with a significance threshold FDR < 5% and fold enrichment ≥ 1.5%. Supporting references confirming GO analysis are reported in the 'References' column.

ISSN: 1474-760X