Skip to main content

Table 3 Alignment statistics for Illumina PE and frag sequencing libraries

From: Whole exome capture in solution with 3 Gbp of data

 

Illumina Frag

Illumina PE

Total reads aligned

33,524,973

37,832,835

Total data aligned (Gbp)

2.51

2.84

Reads on target (%)

67.62

78.0

Duplicate reads (%)

30.97

8.3

Mean coverage (X)a

24

52

Median coverage (X)a

20

40

Targets hit (%)

99.36

99.6

Bases ≥1× Coverage (%)

96.39

98.9

Bases ≥10× Coverage (%)a

71.33

90.8

Bases ≥20× Coverage (%)a

51.23

76.9

  1. aCalculated after duplicate read removal.