Figure 5

Expression of BACE1 , BACE1 -AS and miR-485-5p in Alzheimer's disease-affected individuals. (a) Expression of BACE1, BACE1-AS and miR-485-5p transcripts were measured in parietal lobe and cerebellum of five subjects with Alzheimer's disease and five normal elderly individuals. miR-485-5p was down-regulated by 30% in parietal lobe and 60% in cerebellum of Alzheimer's disease patients compared to control individuals. BACE1-AS as well as BACE1 transcripts were up-regulated in both cerebellum and parietal lobe (unpaired t-test with Welch's correction: ns = not significant; *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001). (b) Expression of BACE1, BACE1-AS and miR-485-5p transcripts were measured in four regions of the brain of 35 Alzheimer's disease patients and 35 control individuals. Not all regions were available from all cases; a total of 120 RNA samples from superior frontal gyrus, entorhinal cortex, hippocampus and cerebellum were tested. miR-485-5p was significantly down-regulated in entorhinal cortex and hippocampus of Alzheimer's disease subjects, but not altered in cerebellum nor in superior frontal gyrus. BACE1-AS, and to a lesser degree BACE1, transcripts were up-regulated in all four regions. However, the increase in BACE1 mRNA was not statistically significant in cerebellum and hippocampus (unpaired t-test with Welch's correction: ns = not significant; *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001)