Figure 3

BACE1 -AS masks the binding site for miR485-5p on BACE1 mRNA. (a) Over-expression (O/E) of miR485-5p significantly reduces BACE1 protein levels in HEK293T C3 cells. BACE1 protein was measured by EFC protein quantification technology (DiscoveRx). LNA-antimir-485-5p, a sequence complementary to the mature miR-485-5p, blocks the effect of miRNA on BACE1 protein expression. LNA-antimiR-485 increases BACE1 protein levels by blocking endogenous miR-485-5p. Each treatment consists of 24 biological repeats and error bars represent standard error of means (SEM; **P-value < 0.01 and ***P-value < 0.001). (b) Simultaneous over-expression of miR-485-5p and BACE1-AS can effectively block the observed BACE1 protein reduction caused by miR-485-5p alone. This indicates that the two ncRNAs can compete for the same binding site on BACE1 mRNA. Each treatment consists of 24 biological repeats and error bars represent SEM (**P-value < 0.01).