Pharmacological dissection of transcriptional networks from the drug-induced gene expression profile. Microarray results are shown as heat maps that include drug-responsive genes with genome-wide significance (Figure 2a). Colored rectangles represent transcript abundance and are labeled below the heat map. Each row contains the mean value from three independent array replicates, where samples from two mice were pooled and used for each microarray. The intensity of the color is proportional to the standardized values (between -2 and 2) from each microarray, as indicated on the bar below the cluster images. The names of enzyme inhibitors or receptor antagonists (inhibitor/antagonist) are indicated on the left. The time scheme of each experiment (a-g) is presented on the right. The arrow indicates (two-tailed t-test, P < 0.05) up- or down-regulation of the expression of a particular gene in comparisons between the drug plus vehicle and saline plus vehicle groups (upper row on each heat map) or drug plus inhibitor/antagonist and drug plus vehicle groups (bottom row). The overall influence was measured as a percentage of inhibition of the drug-induced transcriptional response, with 0% representing no effect and 100% representing complete inhibition. The statistical significance of influence was measured as a comparison of the mean fold change between the drug plus inhibitor/antagonist and saline plus vehicle versus drug plus vehicle and saline plus vehicle groups. The level of significance was measured using a two-tailed t-test: *P < 0.05; **P < 0.01; ***P < 0.001. CRF, corticotrophin-releasing factor; HDAC, histone deacetylase.