Parameter | SFP | RAD tagging | RAD sequencing | RSTA |
---|---|---|---|---|
Marker type | SNPs and indels | Restriction cut site polymorphisms | Sequence data: SNPs next to restriction cut sites | Restriction cut site polymorphisms: distinguishes SNPs and indels |
Number of loci surveyed | 92,924 | 19,200 (elements on an enriched RAD-tag microarray designed from stickleback) | 26 nucleotides at 41,622 RAD tags | 50,935 |
Number of polymorphisms identified (informative marker rate) | 3,806 (4% at a 5% false discovery rate cutoff) | 1,990 (10% at a two-fold signal difference cutoff) | Approximately 13,000 (31%) | 12,431 (24%) |
False discovery rate | 3% (117 out of 121 confirmed correct by sequencing) | 9% (20 out of 22 confirmed correct by sequencing) | Not reported | <1% (113 out of 114 confirmed correct by sequencing) |
Platform | Custom high-density oligonucleotide array (Affymetrix), 25 bp oligo | cDNA or genomic tiling array (in house synthesis) | Illumina sequencing | Custom high-density oligonucleotide array (Agilent), 50 bp oligo |
Prior information required | EST, 454 or genome sequence | EST or RAD-tag library for array synthesis | EST or genome sequence to map short sequence reads | EST, 454 or genome sequence |
Polymorphism identification | Hybridization signal difference among study individuals | Hybridization signal difference between two study individuals | Custom Perl scripts for sequence alignment | Genotype clusters across all study individuals |
Individual genotype data | No | No | No | Yes |
Organisms studied | Yeast, Arabidopsis, Anopheles, several seed plantsa | Drosophila, stickleback, zebrafish, Neurospora | Neurospora | Purple sea urchin |