Polymorphic restriction cut site in pyruvate kinase muscle isozyme across 20 individuals. (a) RSTA array log ratio data separate genotypes of individuals sampled. Cool colored circles represent individuals from Boiler Bay, Oregon; warm colored triangles represent individuals from San Diego, California. The data for each individual are in triplicate. (b) Individual genotypes confirmed by restriction digest gels. Lane 1 is an undigested PCR fragment for size reference, while lanes 2 to 10 are treated with the restriction enzyme; lanes 2, 3, 5, 6, 9, and 10 are from heterozygous individuals; lane 4 is from an individual homozygous for the cut site; lanes 7 and 8 are individuals homozygous for a mutation in the cut site. (c) Genotype data resulting from RSTA can be used to look for differences across populations.