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Table 2 Description of assemblies, Illumina reads and performance of IMAGE

From: Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps

  Statistics of draft assembly Illumina reads used IMAGE performance
Organisms Read type Size (Mb) N50a(kb) Read length Coverageb Insert sizec Total gapsd Gaps closed
Salmonella enterica 1 454 4 117 54 295 225 73 49 (68%)
Salmonella enterica 2 Illumina 4 87 54 274 220 233 194 (83%)
Clostridium difficile 454 4 76.5 54 331 249 118 55 (47%)
Bordetella bronchiseptica 454 5 5 54 420 321 918 397 (43%)
Plasmodium berghei e Cap+454+Illumina 18.5 189 76 140 130 156 71 (46%)
Leishmania donovani 454 30 10 76 83 176 3,826 1,768 (46%)
Echinococcus multilocularis Cap+454 107 108 75 126 310 1,676 895 (53%)
Schistosoma mansoni Cap 307 24 108 30 219 25,214 13,771 (55%)
  1. aThe minimum contig length cutoff to include contigs in a given assembly to have 50% of total assembled sequence. bEstimated coverage is calculated as Length of Illumina read × Number of reads/Assembled genome size. cInsert size is defined as the averaged physical distance between two sequenced fragments that were unambiguously aligned against the contig sequence of the initial assembly. dGap is defined as the region between contigs within a scaffold that has no sequence information. eThe core set of Plasmodium berghei contigs that were aligned to the closely related reference sequence of Plasmodium chabaudi. Cap, capillary reads.