Figure 3
From: Optimized design and data analysis of tag-based cytosine methylation assays
HELP-tagging analysis workflow. The analysis workflow for HELP-tagging data is illustrated. Only sequence reads that contain the adapter sequence and map to a single or ≤ 10 sites are retained, the latter repetitive sequences distributed by weighting among the matched loci. Potential polymorphic loci are annotated. Normalization of HpaII by MspI using the angle calculation described in the previous figure is performed and files are generated for genome browser visualization. UCSC, University of California Santa Cruz.