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Table 2 Splice junctions and insertions reported by SplitSeek with anchor length 22

From: Global and unbiased detection of splice junctions from RNA-seq data

 

Oocyte 1

Oocyte 2

Oocyte 1+2

Number processed reads

11,565,660

23,488,851

35,054,511

Predicted splice junctions

17,397

23,703

31,532

Within chromosome

16,205 (93.1%)

21,495 (90.7%)

27,957 (88.7%)

Within 1 Mb

16,128 (92.7%)

21,374 (90.2%)

27,757 (88.0%)

Within 100 kb

16,094 (92.5%)

21,323 (90.0%)

27,685 (87.8%)

Match to a RefSeq exon-exon boundarya

14,264 (82.0%)

18,139 (76.5%)

23,235 (73.7%)

Expected false within 1 Mb (FDR)

12.9 (8.0·10-4)

17.6 (8.2·10-4)

23.4 (8.4·10-4)

Expected false within 100 kb (FDR)

1.3 (8.0·10-5)

1.8 (8.2·10-5)

2.3 (8.4·10-5)

Predicted insertions

275

553

834

  1. aEach of the exon boundaries located within 5 bp of predicted junction.