Figure 3

The human-specific pseudogene of the major urinary protein. A G-to-A nucleotide substitution (with the reverse highlight) at the donor site of the second intron (delineated by the underlined splicing sites) abolishes the ORF of the coding sequence. The sequence conservation is clearly discernable from the multiple sequence alignment of polypeptide sequences translated from partial exonic sequences upstream and downstream of the splicing junction of MUP from 24 species.