Figure 2From: A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454Barcode adapter design. Validated barcode sequences are added to the end of the 454 A adapter via DNA synthesis (Integrated DNA Technology). The lengths of each portion of the adapter and the approximate length of the insert are indicated. Validated barcodes are exactly 11 flows in length and range from 5 to 8 bases. emPCR, emulsion PCR.Back to article page