Candidate genes for alcohol preference identified by expression profiling in alcohol-preferring and -nonpreferring reciprocal congenic rats

Table 2 Quantitative RT-PCR confirmation

		Ratio of expression (iP vs P.NP)^a
		Nucleus accumbens		Amygdala		Frontal cortex		Hippocampus		Caudate putamen
Affymetrix ID	Gene symbol	Microarray	qRT-PCR	Microarray	qRT-PCR	Microarray	qRT-PCR	Microarray	qRT-PCR	Microarray	qRT-PCR
1368358_a_at	Ptprr	2.22	2.28	2.47	2.71	2.17	1.85	2.42	2.77	1.98	2.28
1395714_at	Copg2 IT	-3.97	-2.45	-28.29	-1.73	-31.36	-1.61	-4.57	-2.12	-20.13	-1.23
1394939_at	Ppm1k	-2.05	1.30	-1.74	-1.62	-2.79	-2.54	-1.86	-3.12	-2.39	-2.49
1379275_at	Snx10	1.67	-1.16	2.18	1.68	1.94	1.15	1.69	2.42	2.02	1.64
1380094_a_at	Zfp212	1.30	1.54	1.22	-1.04	1.21	1.19	1.28	1.58	1.43	1.86
1367734_at	Akr1b1	1.22	1.13	1.12	1.30	1.27	1.58	1.16	1.06	1.25	1.15
1379480_at	Dgki	1.23	2.72	1.13	-1.26	1.17	-2.97	1.26	1.11	1.25	-1.71
1370007_at	Pdia4	1.24	1.57	1.34	-1.01	[1.14]	1.05			1.36	-1.13
1367977_at	Snca					-1.11	-1.22	[1.07]	1.05	-1.12	-1.09
1387154_at	Npy					[-1.11]	1.01			[-1.08]	-1.20

^aA positive number indicates the ratio of the expression level of iP/P.NP; a negative number indicates the ratio of expression level of P.NP/iP. Bold numbers in the microarray columns indicate expression is significantly different at FDR <0.05; square brackets indicate FDR between 0.05 and 0.25. qRT-PCR value is an average of six technical replicates.

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