Activity map of the tammar X chromosome shows that marsupial X inactivation is incomplete and escape is stochastic

Table 1 Quantitative analysis of male fibroblast RNA-FISH data

Genes on BACs or fosmids	Percent male nuclei with one signal
*G6PD* , *IKBKG*	95%
TMLHE	96%
RPL10X	98%
*MECP2X* , *IRAK1* , *TMEM187*	99%
HCFC1X	99%
AR	94%
PSMD10	98%
STAG2	95%
HPRT, PHF6X	95%
F9	0%
UPF3B	99%
PGK1	98%
*ATRX*	98%
RBMX	95%
UBA1, RBM10	98%
TBC1D25, GATA1	98%
GATA1, WDR13	94%
GLA, GLRA4	98%
PLP1	0%
KDM5C	96%
*HUWE1X*	97%
AKAP4	99%
LRCH2	96%
WDR44	94%
AMOT	95%

Frequency of nuclei with single signal for X-borne loci investigated in this study. The efficiency of RNA-FISH hybridization for each locus is based on at least 100 male nuclei with two signals for the control autosomal gene, and therefore diploid. Genes highlighted in bold were used in sequential RNA-DNA FISH experiments.

ISSN: 1474-760X