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Figure 1 | Genome Biology

Figure 1

From: Caenorhabditis elegans chromosome arms are anchored to the nuclear membrane via discontinuous association with LEM-2

Figure 1

Chromosome arms are associated with the nuclear membrane. (a) Immunofluorescence analysis of C. elegans embryos with anti-LEM-2 antibodies (green), and the mAb414 antibody, which labels nuclear pore complexes (red). In the merged image, DNA stained by DAPI is shown in blue. The top row is wild-type N2 embryos; the bottom row is the lem-2 null mutant embryos. The arrowhead indicates the nucleus shown more closely in (b). (b) Enlarged image of the nucleus indicated by arrowhead in (a). (c) LEM-2 or negative control ChIP-chip (Array) or ChIP-seq (Seq) profiles. LEM-2* and LEM-2† indicate antibody Q3891 and Q4051, respectively. Vertical bars in the tracks indicate average ChIP-chip signals (MA2C scores) or ChIP-seq signals (z-scores of (IP - input)) in 5-kb windows. The y-axis range is -2 to 2. (d,e) LEM-2 ChIP-chip signals (5-kb window MA2C scores), recombination rate (interpolated genetic position of genes in centimorgans (cM)), and coverage of repetitive sequences in 50-kb windows are shown on chromosomes III (d) and X (e). The other chromosomes are shown in Figure S2c in Additional file 1. Dashed lines indicate the edges of LEM-2 domains as judged by visual inspection.

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