Figure 3

Comparison of coverage bias. (a) Coverage distribution across the E. coli genome with transposase (blue), sonication (red), and endonuclease (green) methods (solid lines) and replicates (dotted lines), normalized for total sequencing depth. (b) Coverage distribution across the PA1 and CRW10 bacteriophage genomes with transposase (blue), nebulization (red), and endonuclease (green) methods (dotted lines represent replicate libraries). (c) G+C bias for E. coli was assessed by calculating G+C content of the reference in 500 bp bins and plotting the coverage in each for transposase (blue), sonication (red), and endonuclease (green) methods, all of which show an approximately equivalent bias against the extremes.