Figure 2

Haploinsufficiency for Trim28 caused by a splice site mutation. (a) Sequence chromatograms show that MommeD9^-/+ mice have a T to C mutation 2 bp into intron 13 of Trim28. (b) The mutation is expected to prevent splicing of intron 13 causing an in-frame premature stop codon. The splice acceptor site is shown in black. (c) Northern and western analysis of Trim28 mRNA and protein show that MommeD9^-/+ mice have a reduced dosage of Trim28. Error bars indicate + SEM.