Table 6 Quality assessment of the filtered putative SNPs identified from the catfish ESTs based on the number of sequences per contig and the sequence frequencies of the minor alleles
Number of sequences in the contig | Number of contigs with SNPs | Number of SNPs | SNP rate (per kb) |
|---|---|---|---|
2 (1:1) | 16,567 | 96,565 | 5.2 |
3 (2:1) | 8,374 | 86,686 | 10.8 |
4 (3:1) | 5,136 | 71,155 | 13.0 |
Subtotal | 30,077 | 254,406 | 8.0 |
4 (2:2) | 1,528 | 5,008 | 0.9 |
5-6 (2) | 3,099 | 13,725 | 2.0 |
7-8 (3) | 805 | 2,659 | 0.7 |
9-12 (4) | 730 | 2,376 | 0.5 |
13-20 (5) | 629 | 2,307 | 0.6 |
21-30 (5) | 628 | 2,864 | 1.3 |
31-50 (6) | 730 | 5,052 | 3.0 |
51-100 (6) | 542 | 6,379 | 6.0 |
101-500 (6) | 316 | 6,580 | 13.4 |
>500 | 31 | 1,644 | 15.0 |
Subtotal | 9,038 | 48,594 | 1.6 |
Total | 39,115 | 303,000 | 7.7 |