Restoration of proliferative chromosome position after restimulation of G
cells. The relocation of chromosomes to their proliferative nuclear location takes 24+ hours for chromosome 10 and 36 hours for chromosome 18. Proliferating cells (a, g, m) were placed in low serum (0.5%) for 7 days (b, h, n) and then restimulated to enter the proliferative cell cycle with the readdition of high serum. Samples were taken at 8 hours (c, i, o), 24 hours (d, j, p), 32 hours (e, k, q), and 36 hours (f, l, r) after restimulation. The graphs display the normalized distribution of chromosome signal in each of the five shells. Shell 1 is the nuclear periphery, and shell 5 is the innermost region of the nucleus. The solid squares represent a significant difference (P < 0.05) for that shell when compared with the equivalent shell for the time 0 data (G0 data) for the erosion analysis.