Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae

Table 2 Genes essential for maintenance of mtDNA

Genes encoding components involved in mtDNA metabolism
*YKL114c	APN1	Involved in repair of DNA damage; located in nucleus and mitochondria
YLR304c	ACO1	Aconitase; also independently required for mtDNA maintenance
YML061c	PIF1	DNA helicase; active in nucleus and mitochondria
YOL095c	HMI1	Mitochondrial inner membrane localized DNA helicase
YOR330c	MIP1	Catalytic subunit of the mitochondrial DNA polymerase
Genes encoding components involved in mitochondrial transcription and translation
*YBR268w	MRPL37	Mitochondrial ribosomal protein
*YCR024c	SLM5	Mitochondrial asparaginyl-tRNA synthetase
*YDR175c	RSM24	Mitochondrial ribosomal protein of the small subunit
YHR168w	MTG2	Associates with mitochondrial ribosome; possible role in ribosome assembly
*YKL169c		Dubious ORF; partially overlaps with MRPL38
YMR228w	MTF1	Mitochondrial RNA polymerase specificity factor
Genes encoding components involved in oxidative phosphorylation
*YDR079w	PET100	Specifically facilitates the assembly of cytochrome c oxidase
YPL078c	ATP4	Subunit b of the stator stalk of mitochondrial F₁F₀ ATP synthase
Other genes
*YGL240w	DOC1	Required for the ubiquitination activity of the anaphase promoting complex
*YLR091w	RRG5	Unknown function
YOR211c	MGM1	Mitochondrial GTPase involved in fusion

The list indicates systematic and standard names of genes essential for maintenance of newly re-introduced mtDNA in class I pet mutants. The cellular roles of the proteins are indicated according to the Saccharomyces Genome Database [19] or manually annotated. Genes that were previously not known to be essential for maintenance of mtDNA are indicated with an asterisk.

ISSN: 1474-760X