N-BPs modulate DBF4 expression and trafficking. MCF-7 cells were treated with 10-4 M ALE, RIS and IBA for 72 h, and nuclear and cytoplasmic extracts were subjected to SDS-PAGE. (a) Representative western blot (WB) analysis of DBF4 expression level; actin was used as loading control (Ctrl). (b) MCF-7 cells were fixed and stained for DBF4 (green) after stimulation with 10-4 M ALE, RIS or IBA for 48 h. Nuclei were visualized by propidium iodide (P.I.) counterstaining (red). Scale bar: 20 μm.