Double-fluorescent whole-mount in situ hybridization (DFWIS) of novel regulators and subcellular localization. DFWIS A-L. Ath5 mRNA was detected using TSA-fluorescein (green), and regulator mRNA was visualized using FastRed staining (purple). (a, b) Group 1, activators in RPCs. (c, d) Group 3, activators in RGCs. (e-l) Group 4, repressors in RGCs. (m-r) Cellular localization. BHK21 cells were transfected with GFP-fusion proteins. The upper half of each image shows the single channel including the GFP-fusion protein. The lower half of each image shows an overlay of the GFP-fusion protein (green), DAPI-stained nucleus (blue) and lynd-Tomato stained cell membrane (purple).