Characterization of L1 at 6p22.2. (a) Diagram of L1 at 6p22.2 and the surrounding region. The arrow designates the L1 transcript. Triangles at F and R indicate positions of oligonucleotides 6p22F and 6p22R. Blue boxes indicate exons of the FAM65B gene, with exon number designated. Oligonucleotides FAM65B-1 and FAM65B-2 are indicated. The sequence of the 3' expression tag is provided. (b) Expression of CEPH individuals GM10861 and GM11994 of the L1 at 6P22.2 compared to flanking exons from FAM65B, normalized to HPRT1. Expression is plotted on a logarithmic scale so that levels for both amplicons can be clearly visualized. Error bars represent ± standard deviations from three replicates. Data are representative of two experimental replicates.