Figure 4

Validation of microarray-predicted changes by quantitative RT-PCR (qRT-PCR). (a) Differential expression of 26 genes, 10 up-regulated (bottom bars), 12 down-regulated (top bars) and 4 unchanged (middle bars), was verified by real time RT-PCR using specific primer pairs (Additional data file 11). The represented values were normalized and are expressed relative to β-actin, whose value is fixed arbitrarily to 1, and are means ± standard error of the mean (SEM; n = 3; *P < 0.001; **P < 0.01; ***P < 0.05). (b) Differential expression of eight genes, five up-regulated (IL-6, IL-8, AREG, PTGS2 and EREG), two down-regulated (TNFSF10 and CD24) and one unaffected (β-actin), from cytoplasmic RNA isolated from HeLa cells transfected with either control (white bars), TIA-1 (grey bars), TIAR (dark grey bars) or TIA-1 plus TIAR (black bars) siRNAs was verified as above. The represented values were normalized and are expressed relative to β-actin, whose value is fixed arbitrarily to 1, and are means ± SEM (n = 3; *P < 0.001; **P < 0.01).