Figure 2

Knockdown of TIA proteins activates an acute inflammation-promoting transcriptome. (a) Heat map representation depicting microarray data for genes that are differentially regulated by siRNA-mediated reduction of TIA-1 and TIAR. Mean fold-change values from three independent replicates for each biological condition tested are given for 538 probe sets (horizontal lanes; out of a total of 47,400 sets) that were detectable above background showing at least a 1.5-fold change in expression and were statistically different (P < 0.05) in the two experimental conditions tested. Red and green indicate up- and down-regulation, respectively, relative to control siRNA-transfected HeLa cells. The color scale to the right indicates the magnitude of the fold change (base 2 logarithm) for a particular transcript. (b) Total number of genes whose expression was up- or down-regulated in TIA-1 and TIAR-depleted cells. (c, d) Graphic pie representations of the distribution of up- (c) and down-regulated (d) genes (P < 0.01) using the GO biological process category. (e, f) Graphic pie representations of the distribution of up- (e) and down-regulated (f) genes (P < 0.05) using the KEGG pathway database. In all cases (c-f), percentages shown reflect the portion of total genes that are associated with the biological functions and pathways indicated.