Validation using quantitative PCR. Individual genes with different biological roles were selected from the reward pool (Figure 3a) for qPCR using original RNA from (a) wt+/wt- and (b) mut+/sib+. The qPCR experiment revealed selected genes showed expression changes similar to those seen in the microarray results. The figures show box plots of relative gene expression, where the top and the bottom of each box indicate the 75th and 25th percentiles, respectively, whereas the dotted line represents the median. Asterisks indicate that the probability of the alternative hypothesis (that the difference between sample and control groups is only due to chance (P(H1)) being correct is ≤ 0.05 (see also Materials and methods).