Motifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons

Table 2 Change of Luciferase activity due to multi-species conserved elements in different cell lines

Summary of Rab3A multi-species conserved element (MCE) effects on Luciferase expression in Neuron2a, HEK293 and Hela cell lines. Rab3A MCE1, 2, 4 and 5 were examined individually by fusing into the Luciferase construct harboring either the Rab3A gene promoter (MCE3) or the SV40 promoter. Three cell lines were used: Neuro2a (mouse neuroblastoma), HEK 293 (human embryonic kidney) and Hela (human adenocarcinoma). MCE1 and MCE2 robustly enhanced Luciferase activity in the Neuro2a cell line but not at a comparable level in HEK293 and Hela cells under the control of either the MCE3 or SV40 promoter. MCE4 repressed Luciferase activity in all three cell lines, even under control of the SV40 enhancer. MCE4 also showed a strong positional effect, in which a position upstream of the MCE3 promoter failed to reduce Luciferase expression. MCE5 led to a decrease of Luciferase activity under the SV40 promoter and SV40 enhancer. However, when MCE1 and MCE2 induced Luciferase activity in Neuro2a cells, MCE5 did not have an effect on Luciferase activity. +, twofold increase; ++, fourfold increase; +++, sixfold increase; +/-, no change; - -, fourfold decrease; - - -, sixfold decrease; - - - -, approximately a tenfold decrease.

ISSN: 1474-760X