Functional validation of 14 high scoring MCEs and 10 low scoring MCEs listed in Table 3. Luciferase constructs were generated by inserting each MCE into the Luciferase promoter vector (pGL3p), which contains a SV40 promoter and Luciferase coding sequence. (a-d) The corresponding Luciferase activity was examined in one neuronal cell line, Neuro2a cells (a), and three non-neuronal cell lines, Hela cells (b), HEK293 cells (c) and MEF cells (d). Each construct was tested in triplicate in each set of experiments. Every experiment of all 24 constructs was repeated at least three times. Each Luciferase construct was co-transfected with the Renilla pRLCMV vector for normalization. MCEs that significantly induced Luciferase activity are highlighted with an asterisk (P < 0.05, Student's t-test). The error bars represent the standard deviation of the mean of triplicates used for each construct.