Skip to main content
Figure 2 | Genome Biology

Figure 2

From: Motifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons

Figure 2

Characterization of Rab3A transcripts. (a) Illustration of the murine Rab3A locus in the University of California at Santa Cruz (UCSC) Genome Browser. Two Rab3A transcripts are shown in the top panel, among which the RefSeq transcript is represented as dark blue blocks. (b) Two splicing forms of Rab3A are labeled as LT (longer transcript) and ST (shorter transcript). The 5' UTR of Rab3A in the ST is shown as an unfilled box. The positions of primers used for RT-PCR are represented as solid arrows. Quantitative PCR primers are represented as open arrows and corresponding labels are in brackets. (c) Presence of Rab3A ST and LT examined by RT-PCR from mouse cortex tissues at various developmental stages. Primer set F1/R1 detects ST, primer set F2/R1 detects LT, and primer set F3/R1 reflects the total Rab3A level. β-actin level is used as control for RNA loading. Corresponding maker size is labeled on the right. (d) Percentage of ST and LT in total Rab3A mRNA was measured by quantitative RT-PCR from the same tissues used in (b). Specific primer sets were designed for ST and LT, respectively. A control primer set was used to detect total Rab3A level. All samples were tested simultaneously with the control set and the test set (either ST or LT). The ratio between ST and total RNA is presented as blue bars, and the ratio between LT and total RNA is presented as red bars. The error bars represent the standard deviation of the mean of four replicates used for each stage.

Back to article page