Figure 1

Knockdown of MENε/β ncRNAs leads to disintegration of the paraspeckles. Confocal images of HeLa cells treated either with a control scrambled antisense oligonucleotide (upper panels) or with a MENε/β knockdown antisense oligonucleotide (lower panels). Upper panel: MENε/β ncRNAs (magenta) co-localize to paraspeckles defined by PSF immunofluorescence (green). Lower panel: the paraspeckle-associated PSF signal disappeared when the MENε/β ncRNAs were successfully depleted, indicating that the paraspeckles have disintegrated. Note that the nucleoplasmic PSF signal remains intact. The HeLa cell nuclei were counterstained with DAPI (blue). Scale bar, 10 μm.