Linkage map of S. mansoni based on 210 markers. The map shows all 243 markers assigned to 210 unique positions on the 8 linkage groups; the numbers are map distances in centimorgans. Loci that had 0% recombination with other markers are shown adjacent to the marker used in the construction of the map. For example, marker sc84 on LG1_Chr1 had 0% recombination with both markers sc26 and sc93b. The Z-specific markers on LG2_ChrZ are shown in green. Asterisks (* P < 0.01, ** P < 0.005, *** P < 0.001, **** P < 0.0005, *****P < 0.0001) indicate significance for deviation from Mendelian expectations. Genes with previously known physical positions from fluorescent in situ hybridization are shown in blue with GenBank accession numbers. Blue lines show the scaffolds that match the DNA sequences of these genes in BLAST searches. These six genes add further support to the anchoring of the LG3, LG4, and LG7 to chromosomes 2, 3, and 7, respectively. See Figure 3 for comments on the match with sc3 and Smox1.