Predicted general metabolism and solute transport in T. gammatolerans. (a) Modified Embden-Meyerhof glycolytic pathway. (b) Pyruvate degradation. (c) Pentose phosphate synthesis and carbon dioxide fixation. (d) Pseudo tricarboxylic acid cycle. (e) Amino acid degradation. (f) Oxygen and reactive oxygen species detoxication. The transporters and permeases deduced from the annotatable CDSs are grouped by substrate specificity: anions (blue), amino acids/dipeptides (green), cations (pink), heavy metal or drug (black), carbohydrates (yellow) and unknown (grey). Dashed lines represent pathways not yet experimentally validated in Thermococcales species. Red illustrates proteins only found in T. gammatolerans or shared with T. onnurineus (Mhy1, Mhy2 and F420 dehydrogenase). A detailed legend of Figure 5, including gene ID, is available in Additional data file 5.