Skip to main content

Table 1 Proteins in honeybee queen spermathecal fluid

From: Insights into female sperm storage from the spermathecal fluid proteome of the honeybee Apis mellifera

  Mated Virgin     
PreRelease2 accession Gel LC MS/MS LC MS/MS Seminal fluid Sperm Pred. secret. Protein functional description
GB10467-PA X X   X    Aspartate aminotransferase 2 precursor
GB10973-PA   X X X    Arginine kinase
GB15049-PA    X     Delta-1-pyrroline-5-carboxylate synthetase
GB15171-PA X X    X   Ornithine aminotransferase precursor
GB16218-PA    X     Proline oxidase
GB17641-PA X X X     Alanine aminotransferase 2
GB18844-PA X X X     Glutamate oxaloacetate transaminase 1
GB10133-PA X X      Superoxide dismutase
GB10498-PA X X X     Peroxiredoxin
GB12029-PA X       Glyoxalase domain-containing protein
GB14972-PA X      S Thioredoxin reductase
GB15855-PA X       Thioredoxin-2
GB18955-PA   X X X   S Phospholipid hydroperoxide glutathione peroxidase
GB19380-PA   X X     Thioredoxin peroxidase 1
GB30268-PA X X X     Glutathione s transferase S1
GB12586-PA    X    S Protein disulfide-isomerase precursor
GB12447-PA X       CAP, adenylate cyclase-associated protein 1
GB13596-PA    X     ATP synthase
GB14791-PA    X     ATP synthase subunit
GB15291-PA    X     ATP synthase gamma subunit
GB16485-PA    X     ATP synthase D chain
GB10989-PA X X X     Vacuolar ATPase catalytic subunit A
GB11380-PA X X X     Vacuolar H+ ATPase 44 kDa C subunit
GB12913-PA X X X     Vacuolar proton pump E subunit
GB13499-PA X X X     Vacuolar ATPase subunit G
GB15226-PA X X      Vacuolar ATPase subunit D 1
GB17480-PA X X X     Vacuolar ATPase subunit H
GB17499-PA    X     ADP/ATP translocase
GB19171-PA X X X     Vacuolar ATPase 55 kDa B subunit
GB20017-PA X       Endoplasmic reticulum ATPase
GB10355-PA   X X    S Melittin
GB10695-PA X X X   X   Pyruvate kinase
GB10992-PA X       ATP citrate lyase isoform A
GB11056-PA X X X X    Phosphoglycerate kinase isoform 1
GB11461-PA X       UTP-glucose-1-phosphate uridylyltransferase
GB12488-PA    X     Aconitase
GB12573-PA   X X X    Citrate synthase
GB12741-PA    X     Aldehyde dehydrogenase
GB12949-PA   X X     6-Phosphogluconate dehydrogenase
GB13058-PA   X    X   Dihydroxyacetone kinase 2
GB13237-PA X X X     Phosphogluconate mutase
GB13882-PA X X      L-lactate dehydrogenase
GB13955-PA   X X     N-acetyltransferase 5
GB14517-PA   X X     Isocitrate dehydrogenase
GB14798-PA X X X   X   Glyceraldehyde-3-phosphate dehydrogenase 2
GB14803-PA   X X    S Alpha,alpha-trehalase
GB15039-PA X X X     Enolase
GB15052-PA X X X     Phosphoglyceromutase
GB15463-PA   X    X   Aldolase
GB15543-PA   X   X    Malate/L-lactate dehydrogenases
GB15619-PA X       Transketolase-like
GB15888-PA   X X     Carbonic anhydrase
GB16429-PA X X X X    Glucose-6-phosphate isomerase
GB16464-PA X X X     malate dehydrogenase
GB16951-PA X       Malic enzyme
GB17113-PA X X X     Phosphofructokinase
GB17473-PA X X X X    Triosephosphate isomerase 1
GB18109-PA   X    X   Aldose reductase (NADP+)
GB18727-PA X X X     Malate dehydrogenase
GB19030-PA X X X     Aldo/keto reductase family protein
GB19387-PA   X X   X   Hexokinase A, isoform A
GB19460-PA X X X     Aldolase, isoform F
GB11665-PA   X X X   S Chitinase-like protein
GB11876-PA    X    S LDLa domain containing chitin binding protein
GB16986-PA   X X    S Endochitinase precursor
GB10397-PA    X     Alpha-crystallin
GB10800-PA X       T-complex protein 1
GB10836-PA X       HSP70
GB14758-PA X X X X    Heat shock protein 90
GB14852-PA X X X X    Heat shock protein 8 isoform 1
GB15016-PA X X X     Heat shock protein cognate 3
GB17056-PA X X X     Cyclophilin 1
GB18662-PA    X     Alpha-crystallin, small HSP
GB18969-PA X   X     Heat shock protein 60
GB12818-PA    X     Histone 2A
GB14548-PA   X X    S Deoxyribonuclease II
GB16515-PA X       ATP dependent DNA helicase
GB19247-PA X       Elongation factor 2, isoform 1
GB16568-PA    X X    Cytochrome c oxidase subunit
GB19293-PA X X X     Cytochrome c
GB19729-PA X X     S Cytochrome c
GB11059-PA X       Retinoid- and fatty-acid binding protein
GB15044-PA   X X X    Phosphatidylethanolamine-binding protein
GB14639-PA   X X    S Major royal jelly protein 8
GB16324-PA   X X    S Major royal jelly protein 9
GB12951-PC X X X     14-3-3-like protein
GB15202-PA   X      DJ-1, neuroprotective transcriptional co-activator
GB15582-PA   X X     14-3-3 epsilon
GB16178-PA X       Neuropeptide Y receptor
GB16716-PA    X    S Leucine-rich repeat-containing protein
GB16072-PA X X X     Iron regulatory protein 1B
GB10536-PA    X     Odorant binding protein 14
GB19662-PA   X     S Juvenile hormone binding protein
GB19745-PA   X     S Transferrin
GB10009-PA X X X   X   Tubulin alpha-1 chain
GB10091-PA    X    S Cuticlin-1 precursor
GB10122-PA   X X   X   Tubulin, beta, 2
GB10275-PA X X X X    Tubulin isoform B
GB10514-PA X X X   X   alpha tubulin
GB11282-PA X X X     Moesin isoform D
GB11920-PA X       Tubulin
GB12614-PA   X      Actin
GB13049-PA   X      Tubulin, beta, 2
GB13229-PA X       PDZ and LIM domain protein
GB13999-PA X X X    S Vitellogenin
GB15794-PA    X    S Cuticlin-1 precursor
GB16448-PA   X X X    Annexin IX
GB17673-PA X       Talin-1
GB17681-PA X X X X    Actin-5C isoform 1
GB18917-PA   X X     Cofilin/actin-depolymerizing factor homolog
GB12113-PA    X     Porin
GB14012-PA    X     Phosphate carrier
GB16577-PA    X     Sialin, inorganic phosphate cotransporter
GB11987-PA   X   X    Unknown
GB12562-PA X X X     Hypothetical protein
GB13778-PA X      S Unknown
GB14970-PA X       Muscle-specific protein 300
GB15662-PA X X X X    Unknown
GB17311-PA   X X X   S Unknown
GB17500-PA    X    S Hypothetical protein
GB19255-PA X      S Osiris 14 CG1155-PA
GB30569-PA   X X    S Hypothetical protein
  1. Proteins were identified by MS/MS analysis of one-dimensional gel and gel-free analysis of tryptic peptides from spermathecal fluid extracted from mated and virgin queens. Data are compared to the previous reported analysis of the male seminal fluid and sperm proteomes [19]. Details of the mass spectrometry analysis and detailed matching are shown in Additional data file 1. Significant identification of peptides from a specific bee locus number are shown with an 'X' for gel analyses; the number of proteins predicted to be secreted (Pred. secret.) are shown with an 'S' based on consensus of analysis of amino acid sequences using two different software packages TargetP [52] and IPsort [53].