Figure 4

Comparisons of H3K4me accumulated in wild-type Arabidopsis (Wt, green) and the met1 mutant (light brown). Left: chromosome-level changes in H3K4me, showing the ectopic accumulation of H3K4me in the pericentromeric heterochromatin. Chromosome 5 is shown as an example (Wt, green; met1, light brown). X-axis: chromosome position; y-axis: the percentage of H3K4me on chromosome 5 in the corresponding region (in 100 kb bins). Right: local changes in DNA methylation, H3K4me and transcription in a euchromatic region (top right) and a heterochromatic region (bottom right) on chromosome 5. The five genes shown in the euchromatic region likely encode cellular proteins and their expression patterns are unaffected in the met1 mutant. These are (from left to right): At5g56210, WPP-DOMAIN INTERACTING PROTEIN 2; At5g56220, nucleoside-triphosphatase; At5g56230, prenylated rab acceptor (PRA1) family protein; At5g56240, unknown protein. The six genes shown in the heterochromatic region are all transposon-encoded genes. These are (from left to right): At5g32925, CACTA-like transposase; At5g32950, CACTA-like transposase, At5g32975, similar to En/Spm-like transposon protein; At5g33000, Transposable element gene; At5g33025, gypsy-like retrotransposon; At5g33050, gypsy-like retrotransposon. Note that the overexpression of At5g32950 and At5g33050 is associated with ectopic accumulation of H3K4me.