miRNAs showing major changes in expression between any two stages during development. The number of reads of each miRNA was plotted after normalization (see Materials and methods). miRNAs expressed in (a) high abundance (more than 10,000 reads at any stage) and (b) lower abundance are shown separately. For clarity, miRNAs with fewer than 200 reads are not shown. Emb, embryo; mL, mid-larval stage; yAdult, young adult.