Chromosome association of the cohesin loader and condensin during mitosis. (a) Cell fractionation of a culture progressing through synchronous mitosis after release from cdc25-22 arrest was performed as in Figure 5b. The distribution of the cohesin loader subunit Ssl3-Pk9 was analyzed. (b) As (a), but the chromosomal association of the condensin subunit Cnd2-Pk9 was detected. (c) Cytological comparison of the staining intensities of the cohesin loader subunit Mis4-Pk9, and the condensin subunit Cut14-Pk9, on spread chromosomes. Anaphase cells were identified among cells in other cell cycle stages by two segregating Mis6-GFP signals.