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Table 3 NarL binding sites with questionable annotations

From: MotifAdjuster: a tool for computational reassessment of transcription factor binding site annotations

Gene ID

Gene name

BS

Lit.

Occ.

Shift

Strand

Adj. BS

b0904

focA

AATAAAT

[63]

1

+1

Reverse

TATTTAT

b0904

focA

ATAATGC

[63]

1

+1

Forward

TAATGCT

b0904

focA

ATATCAA

[63]

1

+1

Forward

TATCAAT

b0904

focA

CAACTCA

[63]

1

+1

Forward

AACTCAT

b0904

focA

CATTAAT

[63]

1

+1

Reverse

TATTAAT

b0904

focA

GATCGAT

[63]

1

+1

Reverse

TATCGAT

b0904

focA

GTAATTA

[63]

1

+1

Forward

TAATTAT

b0904

focA

TATCGGT

[63]

1

+1

Reverse

TACCGAT

b0904

focA

TTACTCC

[63]

1

+1

Forward

TACTCCG

b1223

narK

CACTGTA

[64]

0

-

-

-

b1224

narG

TAGGAAT

[64]

1

+1

Reverse

AATTCCT

b4070

nrfA

TGTGGTT

[65]

1

+1

Reverse

TAACCAC

b4123

dcuB

ATGTTAT

[66]

0

-

-

-

  1. Annotated NarL BSs for which MotifAdjuster proposes either to shift the BS or to remove it from the data set. Columns 1 to 3 contain gene ID, gene name, and the BS (as stored in the database). Column 4 indicates the original literature related to this BS. The following three columns (5 through 7) comprise the three possible adjustments suggested by MotifAdjuster, removal, shift, and strand orientation (relative to the target gene). In column 5, a value of 0 indicates that the BS is proposed for removal, and in column 6, a positive (negative) value denotes a shift of the BS to the right (left). Finally, column 8 provides the adjusted BS. Interestingly, we find that the two BSs that are proposed to be removed are not mentioned in the original literature, and in 10 of the 11 cases, the shifted BS is consistent with the BS published in the original literature. In addition, MotifAdjuster also proposes to switch the BS strand in six of the 11 cases.