CG7236 and minibrain show cell line-specific phenotypes. (a) Silencing of the cdc2-related kinase CG7236 in S2R+ cells gives rise to large cells that frequently contain multiple nuclei or a single large nucleus, whereas silencing in BG3-c2 cells has no discernable phenotype. (b) Silencing of the DYRK family kinase minibrain in BG3-c2 cells causes an increase in peripheral actin and an increase in the number of protrusions per cell, whereas silencing in S2R+ cells has no phenotype. Also, the BG3-c2 cells forced to spread by plating on concanavalin A (ConA) exhibit large lamellipodia when in the presence of a non-targeting dsRNA, but not in the presence of mnb dsRNA. (c) Quantification of the mnb RNAi phenotype shows a significant twofold increase in the number of long finger-like protrusions around the cell body. (d) Q-PCR analysis reveals that CG7236 and minibrain are effectively silenced by RNAi reagents in both S2R+ and BG3-c2 cells. Error bars indicate the standard error of the mean.